20 February 2014 Label-free biochemical characterization of bovine sperm cells using Raman microscopy
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The current study relates to a Raman spectroscopy-based method for addressing the problem of sex assessment in mammals. A direct method for sex predetermination in animals is based on the X- and Y-bearing sperm cells sorting before insemination. Our Raman spectroscope allows distinguishing and characterizing the difference between X- and Y-bearing sperm cells by detecting and analyzing their Raman spectra in a non-invasive and non-destructive way.
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A. C. De Luca, A. C. De Luca, S. Managò, S. Managò, M. A. Ferrara, M. A. Ferrara, L. Sirleto, L. Sirleto, R. Puglisi, R. Puglisi, D. Balduzzi, D. Balduzzi, A. Galli, A. Galli, I. Rendina, I. Rendina, P. Ferraro, P. Ferraro, G. Coppola, G. Coppola, "Label-free biochemical characterization of bovine sperm cells using Raman microscopy", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893819 (20 February 2014); doi: 10.1117/12.2039085; https://doi.org/10.1117/12.2039085

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