Analysis of virus infected cell by Raman spectroscopy and transmission electron microscopy

Kamila Moor; Kiyoshi Ohtani; Diyas Myrzakozha; Orik Zhanserkenova; Bibin B. Andriana; Hidetoshi Sato

doi:10.1117/12.2038020

4 March 2014 Analysis of virus infected cell by Raman spectroscopy and transmission electron microscopy

Kamila Moor, Kiyoshi Ohtani, Diyas Myrzakozha, Orik Zhanserkenova, Bibin B. Andriana, Hidetoshi Sato

Proceedings Volume 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry; 893902 (2014) https://doi.org/10.1117/12.2038020
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Raman spectroscopy is a promising tool for detection of virus infection in live cells. In the present study, we demonstrate its feasibility to observe dynamic reaction of the live cell infected by virus. The Raman spectra of the adenovirus infected live cell (293 HEK) are analyzed by comparing with those of control cells. Principal component analysis (PCA) is employed also to analyze the spectra in detail. A band at 1650 cm-1 increases its intensity in the spectra measured at 24 hours after the virus infection. The infection of the virus is also examined by immune-staining and transmission electron microscope (TEM), and the virus infection is confirmed with these method also. It should be noted that the present technique does not require specifying the type of virus in advance.

Citation Download Citation

Kamila Moor, Kiyoshi Ohtani, Diyas Myrzakozha, Orik Zhanserkenova, Bibin B. Andriana, and Hidetoshi Sato "Analysis of virus infected cell by Raman spectroscopy and transmission electron microscopy", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 893902 (4 March 2014); https://doi.org/10.1117/12.2038020

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KEYWORDS

Raman spectroscopy

Principal component analysis

Transmission electron microscopy

Microscopes

Analytical research

Biological research

Luminescence

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