4 March 2014 Investigating the biochemical progression of liver disease through fibrosis, cirrhosis, dysplasia, and hepatocellular carcinoma using Fourier transform infrared spectroscopic imaging
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Abstract
Hepatocellular carcinoma (HCC) is the most common form of primary hepatic carcinoma. HCC ranks the fourth most prevalent malignant tumor and the third leading cause of cancer related death in the world. Hepatocellular carcinoma develops in the context of chronic liver disease and its evolution is characterized by progression through intermediate stages to advanced disease and possibly even death. The primary sequence of hepatocarcinogenesis includes the development of cirrhosis, followed by dysplasia, and hepatocellular carcinoma.1 We addressed the utility of Fourier Transform Infrared (FT-IR) spectroscopic imaging, both as a diagnostic tool of the different stages of the disease and to gain insight into the biochemical process associated with disease progression. Tissue microarrays were obtained from the University of Illinois at Chicago tissue bank consisting of liver explants from 12 transplant patients. Tissue core biopsies were obtained from each explant targeting regions of normal, liver cell dysplasia including large cell change and small cell change, and hepatocellular carcinoma. We obtained FT-IR images of these tissues using a modified FT-IR system with high definition capabilities. Firstly, a supervised spectral classifier was built to discriminate between normal and cancerous hepatocytes. Secondly, an expanded classifier was built to discriminate small cell and large cell changes in liver disease. With the emerging advances in FT-IR instrumentation and computation there is a strong drive to develop this technology as a powerful adjunct to current histopathology approaches to improve disease diagnosis and prognosis.
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Hari Sreedhar, Hari Sreedhar, Mamta Pant, Mamta Pant, Nemencio R. Ronquillo, Nemencio R. Ronquillo, Bennett Davidson, Bennett Davidson, Peter Nguyen, Peter Nguyen, Rohini Chennuri, Rohini Chennuri, Jacqueline Choi, Jacqueline Choi, Joaquin A. Herrera, Joaquin A. Herrera, Ana C. Hinojosa, Ana C. Hinojosa, Ming Jin, Ming Jin, Andre Kajdacsy-Balla, Andre Kajdacsy-Balla, Grace Guzman, Grace Guzman, Michael J. Walsh, Michael J. Walsh, } "Investigating the biochemical progression of liver disease through fibrosis, cirrhosis, dysplasia, and hepatocellular carcinoma using Fourier transform infrared spectroscopic imaging", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390J (4 March 2014); doi: 10.1117/12.2040408; https://doi.org/10.1117/12.2040408
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