4 March 2014 Real-time depth-resolved fiber optic Raman endoscopy for in vivo diagnosis of gastric precancer
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Abstract
Raman spectroscopy represents a unique optical vibrational technique based on the fundamental premise of inelastic light scattering. Raman spectroscopy enables histopathological assessment at the biomolecular level. We have developed a fiber-optic depth-resolved near-infrared (NIR) Raman endoscopy technique integrated with on-line diagnostic algorithms for in vivo real-time epithelial diagnostics under multimodal wide-field imaging (i.e., white light reflectance (WLR), narrow-band imaging (NBI), autofluorescence imaging (AFI)) modalities. A selection of 450 patients who previously underwent Raman endoscopy (n=1900 spectra) was used to render diagnostic models for identifying gastric precancer (i.e., dysplasia) based on probabilistic partial least squares (PLS) - discriminant analysis (DA). The on-line Raman endoscopy technique was tested prospectively on (n=5) patients for real-time in vivo gastric epithelium tissue diagnosis. The fiber-optic confocal Raman endoscopic technique developed could prospectively identify gastric dysplasia in real-time with a sensitivity: 81.3% (61/75) and specificity 88.3% (188/213) on spectrum basis. On lesion basis, all dysplastic lesions were identified. This study successfully demonstrates for the first time the prospective real-time in vivo diagnosis of gastric precancer using depth-resolved Raman endoscopy.
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Mads Sylvest Bergholt, Mads Sylvest Bergholt, Wei Zheng, Wei Zheng, Khek Yu Ho, Khek Yu Ho, Khay Guan Yeoh, Khay Guan Yeoh, Ming Teh, Ming Teh, Jimmy Bok Yan So, Jimmy Bok Yan So, Zhiwei Huang, Zhiwei Huang, } "Real-time depth-resolved fiber optic Raman endoscopy for in vivo diagnosis of gastric precancer", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390M (4 March 2014); doi: 10.1117/12.2039552; https://doi.org/10.1117/12.2039552
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