4 March 2014 Fourier transform infrared spectroscopic imaging identifies early biochemical markers of tissue damage
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Abstract
Fourier Transform Infrared (FT-IR) spectroscopic imaging can allow for the rapid imaging of tissue biochemistry in a label-free and non-perturbing fashion. With the rapid adoption of new minimally invasive surgery (MIS) technologies over the last 20 years, adequate skill to safely and effectively use these technologies may not be achieved and risk of undue physical pressure being placed on tissues is a concern. Previous work has demonstrated that a number of histological stains can detect tissue damage, however, this process requires the initiation and progression of a signaling cascade that results in the epitope of interest being expressed. We proposed to identify the early biochemical markers associated with physical tissue damage from applied forces, thus not requiring transcriptional and translational protein synthesis as traditional immunohistochemistry does. To demonstrate that FT-IR can measure biochemical changes in tissues that have undergone physical force, we took ex-vivo lamb’s liver that had been freshly excised and applied varying levels of physical pressure (0kPa to 30kPa). Tissues were then formalin-fixed, paraffin-embedded, and sectioned on to glass for H and E staining to identify damage and on to an IR slide for FT-IR imaging. Regions of interest containing hepatocytes were identified and average FT-IR spectra were extracted from the damaged and undamaged livers. FT-IR spectra showed clear biochemical changes associated with tissue damage. In addition, chemical changes could be observed proceeding histological changes observed when using conventional staining approaches.
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Vishal K. Varma, Vishal K. Varma, Samuel Ohlander, Samuel Ohlander, Peter Nguyen, Peter Nguyen, Christopher Vendryes, Christopher Vendryes, Sujeeth Parthiban, Sujeeth Parthiban, Blake Hamilton, Blake Hamilton, M. Chad Wallis, M. Chad Wallis, Andre Kajdacsy-Balla, Andre Kajdacsy-Balla, Blake Hannaford, Blake Hannaford, Thomas Lendvay, Thomas Lendvay, James M. Hotaling, James M. Hotaling, Michael J. Walsh, Michael J. Walsh, } "Fourier transform infrared spectroscopic imaging identifies early biochemical markers of tissue damage", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390T (4 March 2014); doi: 10.1117/12.2040282; https://doi.org/10.1117/12.2040282
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