4 March 2014 Multimodal fiber probe spectroscopy for tissue diagnostics applications: a combined Raman-fluorescence approach
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Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnosing melanocytic lesions in a good agreement with common routine histology. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. Further investigations were performed on colon and brain tissue samples in order to have a benchmark for diagnosing a broader range of tissue lesions and malignancies. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.
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Riccardo Cicchi, Riccardo Cicchi, Suresh Anand, Suresh Anand, Susanna Rossari, Susanna Rossari, Alessandro Sturiale, Alessandro Sturiale, Flavio Giordano, Flavio Giordano, Vincenzo De Giorgi, Vincenzo De Giorgi, Vincenza Maio, Vincenza Maio, Daniela Massi, Daniela Massi, Gabriella Nesi, Gabriella Nesi, Anna Maria Buccoliero, Anna Maria Buccoliero, Francesco Tonelli, Francesco Tonelli, Renzo Guerrini, Renzo Guerrini, Nicola Pimpinelli, Nicola Pimpinelli, Francesco Saverio Pavone, Francesco Saverio Pavone, "Multimodal fiber probe spectroscopy for tissue diagnostics applications: a combined Raman-fluorescence approach", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390U (4 March 2014); doi: 10.1117/12.2037024; https://doi.org/10.1117/12.2037024

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