4 March 2014 Raman study of analysis for the states of maturation of neural cell
Author Affiliations +
Abstract
The combination of confocal micro-Raman spectroscopy and multivariate analysis is carried out for analysis of maturation of neurons. This study suggests that Raman data reflects the stages of neural maturation which relates with the expression of new neural function such as spontaneous activity. Neurons obtained from a hippocampus of rat embryos are cultured in a dish with quartz bottom. According to the previous electrophysiological study, matured neural cell network showed regulated pulsation with interval of several seconds without any stimulation. It suggested that alterations in the molecular composition took place in the cell. The Raman measurements are carried out to observe this alteration along with the maturing process of neurons. The spectra of live neural cells measured after 2, 8, 15, 30, 45, 60, 75, 90, 105 and 120 days of culturing are analyzed by principal component analysis (PCA). The result shows several groups suggesting the maturation scheme which is observed by the electrophysiological studies. It demonstrates that the maturation process of neural cells can be monitored by Raman spectroscopy.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Kosuke Hashimoto, Kosuke Hashimoto, Suguru N. Kudoh, Suguru N. Kudoh, Hidetoshi Sato, Hidetoshi Sato, } "Raman study of analysis for the states of maturation of neural cell", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 893914 (4 March 2014); doi: 10.1117/12.2039073; https://doi.org/10.1117/12.2039073
PROCEEDINGS
6 PAGES


SHARE
Back to Top