Enhancing the depth of tissue microscope imaging using two-photon excitation of the second singlet state of fluorescent agents

Yang Pu; Lingyan Shi; Sebastião Pratavieira; R. R. Alfano

doi:10.1117/12.2035334

17 March 2014 Enhancing the depth of tissue microscope imaging using two-photon excitation of the second singlet state of fluorescent agents

Yang Pu, Lingyan Shi, Sebastião Pratavieira, R. R. Alfano

Author Affiliations +

Proceedings Volume 8940, Optical Biopsy XII; 894017 (2014) https://doi.org/10.1117/12.2035334
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Increasing the depth to image inside tissue is critical in biomedicine. Two-photon (2P) excitation of the second singlet (S₂) state of a group of fluorescent agents with near infrared emission, Chlorophyll a (Chl a) and Indocyanine green (ICG), is used to extend the optical imaging regime of 2PM into “tissue optical window” for deep tissue penetration. The fast nonradiative from S₂ to S₁ yields both emission and absorption wavelengths in the therapeutic window. The salient feature is to place both the 2P excitation and emission wavelengths of the imaging agents falling into the “tissue optical window”. As a first step to achieve deeper optical imaging, Chl a and ICG are investigated and demonstrated as imaging agents for 2P S₂ excitation microscope image.

Citation Download Citation

Yang Pu, Lingyan Shi, Sebastião Pratavieira, and R. R. Alfano "Enhancing the depth of tissue microscope imaging using two-photon excitation of the second singlet state of fluorescent agents", Proc. SPIE 8940, Optical Biopsy XII, 894017 (17 March 2014); https://doi.org/10.1117/12.2035334

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available