Laser-induced fluorescence spectroscopy in tissue local necrosis detection

Ondrej Cip; Zdenek Buchta; Adam Lesundak; Antonin Randula; Bretislav Mikel; Josef Lazar; Lenka Veverkova

doi:10.1117/12.2052637

13 March 2014 Laser-induced fluorescence spectroscopy in tissue local necrosis detection

Ondrej Cip, Zdenek Buchta, Adam Lesundak, Antonin Randula, Bretislav Mikel, Josef Lazar, Lenka Veverkova

Proceedings Volume 8941, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications; 89411D (2014) https://doi.org/10.1117/12.2052637
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The recent effort leads to reliable imaging techniques which can help to a surgeon during operations. The fluorescence spectroscopy was selected as very useful online in vivo imaging method to organics and biological materials analysis. The presented work scopes to a laser induced fluorescence spectroscopy technique to detect tissue local necrosis in small intestine surgery. In first experiments, we tested tissue auto-fluorescence technique but a signal-to-noise ratio didn’t express significant results. Then we applied a contrast dye - IndoCyanine Green (ICG) which absorbs and emits wavelengths in the near IR. We arranged the pilot experimental setup based on highly coherent extended cavity diode laser (ECDL) used for stimulating of some critical areas of the small intestine tissue with injected ICG dye. We demonstrated the distribution of the ICG exciter with the first file of shots of small intestine tissue of a rabbit that was captured by high sensitivity fluorescent cam.

Citation Download Citation

Ondrej Cip, Zdenek Buchta, Adam Lesundak, Antonin Randula, Bretislav Mikel, Josef Lazar, and Lenka Veverkova "Laser-induced fluorescence spectroscopy in tissue local necrosis detection", Proc. SPIE 8941, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications, 89411D (13 March 2014); https://doi.org/10.1117/12.2052637

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