Dose dependent translocations of fluorescent probes of PIP2 hydrolysis in cells exposed to nanosecond pulsed electric fields

Gleb P. Tolstykh; Melissa Tarango; Caleb C. Roth; Bennett L. Ibey

doi:10.1117/12.2042092

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13 March 2014 Dose dependent translocations of fluorescent probes of PIP₂ hydrolysis in cells exposed to nanosecond pulsed electric fields

Gleb P. Tolstykh, Melissa Tarango, Caleb C. Roth, Bennett L. Ibey

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Proceedings Volume 8941, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications; 89411T (2014) https://doi.org/10.1117/12.2042092
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Previously, it was demonstrated that small nanometer-sized pores (nanopores) are preferentially formed after exposure to nanosecond pulsed electric fields (nsPEF). We have reported that nanoporation of the plasma membrane directly affects the phospholipids of the cell membrane, ultimately culminating in phosphatidylinositol_4,5- bisphosphate (PIP₂) intracellular signaling. PIP₂, located within the internal layer of the plasma membrane, plays a critical role as a regulator of ion transport proteins, a source of second messenger compounds, and an anchor for cytoskeletal elements. In this proceeding, we present data that demonstrates that nsPEFs initiate electric field dose-dependent PIP₂ hydrolysis and/or depletion from the plasma membrane through the observation of the accumulation of inositol_1,4,5-trisphosphate (IP3) in the cytoplasm and the increase of diacylglycerol (DAG) on the inner surface of the plasma membrane. The phosphoinositide signaling cascade presented here involves activation of phospholipase C (PLC) and protein kinase C (PKC), which are responsible for a multitude of biological effects after nsPEF exposure. These results expand our current knowledge of nsPEF induced physiological effects, and serve as a basis for development of novel tools for drug independent stimulation or modulation of different cellular functions.

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Gleb P. Tolstykh, Melissa Tarango, Caleb C. Roth, and Bennett L. Ibey "Dose dependent translocations of fluorescent probes of PIP₂ hydrolysis in cells exposed to nanosecond pulsed electric fields", Proc. SPIE 8941, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications, 89411T (13 March 2014); https://doi.org/10.1117/12.2042092

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