Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry

Liang Gao; Chi Zhang; Chiye Li; Lihong Wang

doi:10.1117/12.2038569

3 March 2014 Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry

Liang Gao, Chi Zhang, Chiye Li, Lihong Wang

Proceedings Volume 8943, Photons Plus Ultrasound: Imaging and Sensing 2014; 894309 (2014) https://doi.org/10.1117/12.2038569
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here we present a technique – fluorescence-assisted photoacoustic thermometry (FAPT) – for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing.

Citation Download Citation

Liang Gao, Chi Zhang, Chiye Li, and Lihong Wang "Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 894309 (3 March 2014); https://doi.org/10.1117/12.2038569

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available