3 March 2014 Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry
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Abstract
Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here we present a technique – fluorescence-assisted photoacoustic thermometry (FAPT) – for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing.
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Liang Gao, Liang Gao, Chi Zhang, Chi Zhang, Chiye Li, Chiye Li, Lihong Wang, Lihong Wang, "Intracellular temperature mapping with fluorescence-assisted photoacoustic thermometry", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 894309 (3 March 2014); doi: 10.1117/12.2038569; https://doi.org/10.1117/12.2038569
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