3 March 2014 DMD-based random-access optical-resolution photoacoustic microscopy
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Abstract
The scanning mechanism is a major technical focus in optical-resolution photoacoustic microscopy. Flexible scanning access with fast scanning speed is desired to monitor biological and physiological dynamics with high temporal resolution. We developed random-access optical-resolution photoacoustic microscopy (RA-OR-PAM) using a digital micromirror device (DMD). Each micromirror on the DMD can be independently controlled, allowing imaging of regions of interest with arbitrary user-selected shapes without extraneous information. A global structural image is first acquired, and the regions of interest are selected. The laser beam then scans these regions exclusively, resulting in a faster frame rate than in a conventional raster scan. This system can rapidly scan arbitrarily shaped regions of interest with a lateral resolution of 3.6 μm within a 40×40 μm2 imaging area, a size comparable to the focal spot size of a 50 MHz ultrasound transducer. We demonstrated the random-access ability of RA-OR-PAM by imaging a monolayer of red blood cells. This system was then used to monitor blood flow in vivo within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 13.3 times.
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Jinyang Liang, Jinyang Liang, Yong Zhou, Yong Zhou, Amy W. Winkler, Amy W. Winkler, Lidai Wang, Lidai Wang, Konstantin I. Maslov, Konstantin I. Maslov, Chiye Li, Chiye Li, Lihong V. Wang, Lihong V. Wang, "DMD-based random-access optical-resolution photoacoustic microscopy", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 894312 (3 March 2014); doi: 10.1117/12.2036496; https://doi.org/10.1117/12.2036496
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