High-resolution raster scan optoacoustic mesoscopy of genetically modified drosophila pupae

Murad Omar; Jérôme Gateau; Vasilis Ntziachristos

doi:10.1117/12.2036185

3 March 2014 High-resolution raster scan optoacoustic mesoscopy of genetically modified drosophila pupae

Murad Omar, Jérôme Gateau, Vasilis Ntziachristos

Proceedings Volume 8943, Photons Plus Ultrasound: Imaging and Sensing 2014; 89431F (2014) https://doi.org/10.1117/12.2036185
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Optoacoutic mesoscopy aims to bridge the gap between optoacoustic microscopy and optoacoustic tomography. We have developed a setup for optoacoustic mesoscopy where we use a high frequency, high numerical aperture spherically focused ultrasound transducer, with a wide bandwidth of 25-125 MHz. The excitation is performed using a diode laser capable of >500 μJ/pulse, 1.8ns pulse width, 1.4 kHz pulse repetition rate, at 515 nm. The system is capable to penetrate more than 5 mm with a resolution of 7 μm axially and 30 μm transversally. Using high-speed stages and scanning the transducer in a quasi-continuous mode, a field of view of 2×2 mm₂ is scanned in less than 2 minutes. The system is suitable for imaging biological samples that have a diameter of 1-5 mm; zebrafish, drosophila melanogaster, and thin biological samples such as the mouse ear and mouse extremities. We have used our mesoscopic setup to generate 3- dimensional images of genetically modified drosophila fly, and drosophila pupae expressing GFP from the wings, high resolution images were generated in both cases, in the fly we can see the wings, the legs, the eyes, and the shape of the body. In the pupae the outline of the pupae, the spiracles at both ends and a strong signal corresponding to the location of the future wings are observed.

Citation Download Citation

Murad Omar, Jérôme Gateau, and Vasilis Ntziachristos "High-resolution raster scan optoacoustic mesoscopy of genetically modified drosophila pupae", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89431F (3 March 2014); https://doi.org/10.1117/12.2036185

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available