3 March 2014 Development of tyrosinase-based reporter genes for preclinical photoacoustic imaging of mesenchymal stem cells
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The capability to image stem cells in vivo in small animal models over extended periods of time is important to furthering our understanding of the processes involved in tissue regeneration. Photoacoustic imaging is suited to this application as it can provide high resolution (tens of microns) absorption-based images of superficial tissues (cm depths). However, stem cells are rare, highly migratory, and can divide into more specialised cells. Genetic labelling strategies are therefore advantageous for their visualisation. In this study, methods for the transfection and viral transduction of mesenchymal stem cells with reporter genes for the co-expression of tyrosinase and a fluorescent protein (mCherry). Initial photoacoustic imaging experiments of tyrosinase expressing cells in small animal models of tissue regeneration were also conducted. Lentiviral transduction methods were shown to result in stable expression of tyrosinase and mCherry in mesenchymal stem cells. The results suggest that photoacoustic imaging using reporter genes is suitable for the study of stem cell driven tissue regeneration in small animals.
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Julia Märk, Julia Märk, Karen Ruschke, Karen Ruschke, Hakan Dortay, Hakan Dortay, Isabelle Schreiber, Isabelle Schreiber, Andrea Sass, Andrea Sass, Taimoor Qazi, Taimoor Qazi, Matthias Pumberger, Matthias Pumberger, Jan Laufer, Jan Laufer, "Development of tyrosinase-based reporter genes for preclinical photoacoustic imaging of mesenchymal stem cells", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89433Z (3 March 2014); doi: 10.1117/12.2039260; https://doi.org/10.1117/12.2039260

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