3 March 2014 Photothermal bleaching and recovery analysis in photoacoustic microscopy
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Abstract
A novel method – photoacoustic recovery after photothermal bleaching (PRAP) – is proposed and implemented to study particle dynamics and medium properties at the micron scale via photoacoustic imaging. PRAP is an intuitive way to visualize as well as quantify dynamic processes in many kinds of media. We demonstrate PRAP first in a phantom study, and then in live cells. PRAP provides high signal-to-noise ratio imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic phenomena inside a cell.
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Chiye Li, Chiye Li, Chi Zhang, Chi Zhang, Liang Gao, Liang Gao, Alejandro Garcia-Uribe, Alejandro Garcia-Uribe, Lihong V. Wang, Lihong V. Wang, } "Photothermal bleaching and recovery analysis in photoacoustic microscopy", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89435Q (3 March 2014); doi: 10.1117/12.2040936; https://doi.org/10.1117/12.2040936
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