3 March 2014 Combined optical and mechanical scanning in optical-resolution photoacoustic microscopy
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Abstract
Combined optical and mechanical scanning (COMS) in optical-resolution photoacoustic microscopy (OR-PAM) has provided five scanning modes with fast imaging speed and wide field of view (FOV). With two-dimensional (2D) galvanometer-based optical scanning, we have achieved a 2 KHz B-scan rate and 50 Hz volumetric-scan rate, which enables real-time tracking of cell activities in vivo. With optical-mechanical hybrid 2D scanning, we are able to image a wide FOV (10×8 mm2) within 150 seconds, which is 20 times faster than the conventional mechanical scan in our second-generation OR-PAM. With three-dimensional mechanical-based contour scanning, we can maintain the optimal signal-to-noise ratio and spatial resolution of OR-PAM while imaging objects with uneven surfaces, which is ideal for fast and quantitative studies of tumors and the brain.
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Lei Li, Chenghung Yeh, Song Hu, Lidai Wang, Brian T. Soetikno, Ruimin Chen, Qifa Zhou, K. Kirk Shung, Konstantin I. Maslov, Lihong V. Wang, "Combined optical and mechanical scanning in optical-resolution photoacoustic microscopy", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89435X (3 March 2014); doi: 10.1117/12.2038693; https://doi.org/10.1117/12.2038693
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