27 February 2014 Imaging marine virus CroV and its host Cafeteria roenbergensis with two-photon microscopy
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Proceedings Volume 8944, Biophotonics and Immune Responses IX; 89440E (2014) https://doi.org/10.1117/12.2041121
Event: SPIE BiOS, 2014, San Francisco, California, United States
Abstract
We use two-photon microscopy to monitor the infection process of marine zooplankton, Cafeteria roenbergensis (C.roenbergensis), by Cafeteria roenbergensis virus (CroV), a giant DNA virus named after its host. Here, we image C.roenbergensis in culture by two-photon excited NADH autofluorescence at video-rate (30 frame/s), and the movement of C.roenbergensis is recorded in live videos. Moreover, CroV is stained with DNA dye SYBR gold and recorded simultaneously with this two-photon microscope. We observed the initial infection moment with this method. The result demonstrates the potential use of two-photon microscopy to investigate the fast dynamic interaction between C.roenbergensis with virus CroV. After catching this initial moment, we will freeze the sample in liquid nitrogen for cryo-electron microscopy (EM) study to resolve the virus-host interaction at molecular level. The long-term goal is to study similar fast moving pathogen-host interaction process which could lead to important medical applications.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Bin Cao, Bin Cao, Sayan Chakraborty, Sayan Chakraborty, Wenqing Sun, Wenqing Sun, Seyedmohammadali Aghvami, Seyedmohammadali Aghvami, Matthias G. Fischer, Matthias G. Fischer, Wei Qian, Wei Qian, Chuan Xiao, Chuan Xiao, Chunqiang Li, Chunqiang Li, } "Imaging marine virus CroV and its host Cafeteria roenbergensis with two-photon microscopy", Proc. SPIE 8944, Biophotonics and Immune Responses IX, 89440E (27 February 2014); doi: 10.1117/12.2041121; https://doi.org/10.1117/12.2041121
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