Imaging marine virus CroV and its host Cafeteria roenbergensis with two-photon microscopy

Bin Cao; Sayan Chakraborty; Wenqing Sun; Seyedmohammadali Aghvami; Matthias G. Fischer; Wei Qian; Chuan Xiao; Chunqiang Li

doi:10.1117/12.2041121

27 February 2014 Imaging marine virus CroV and its host Cafeteria roenbergensis with two-photon microscopy

Bin Cao, Sayan Chakraborty, Wenqing Sun, Seyedmohammadali Aghvami, Matthias G. Fischer, Wei Qian, Chuan Xiao, Chunqiang Li

Author Affiliations +

Proceedings Volume 8944, Biophotonics and Immune Responses IX; 89440E (2014) https://doi.org/10.1117/12.2041121
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We use two-photon microscopy to monitor the infection process of marine zooplankton, Cafeteria roenbergensis (C.roenbergensis), by Cafeteria roenbergensis virus (CroV), a giant DNA virus named after its host. Here, we image C.roenbergensis in culture by two-photon excited NADH autofluorescence at video-rate (30 frame/s), and the movement of C.roenbergensis is recorded in live videos. Moreover, CroV is stained with DNA dye SYBR gold and recorded simultaneously with this two-photon microscope. We observed the initial infection moment with this method. The result demonstrates the potential use of two-photon microscopy to investigate the fast dynamic interaction between C.roenbergensis with virus CroV. After catching this initial moment, we will freeze the sample in liquid nitrogen for cryo-electron microscopy (EM) study to resolve the virus-host interaction at molecular level. The long-term goal is to study similar fast moving pathogen-host interaction process which could lead to important medical applications.

Citation Download Citation

Bin Cao, Sayan Chakraborty, Wenqing Sun, Seyedmohammadali Aghvami, Matthias G. Fischer, Wei Qian, Chuan Xiao, and Chunqiang Li "Imaging marine virus CroV and its host Cafeteria roenbergensis with two-photon microscopy", Proc. SPIE 8944, Biophotonics and Immune Responses IX, 89440E (27 February 2014); https://doi.org/10.1117/12.2041121

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available