4 March 2014 Raman microbeam spectrometer noninvasively measures biomoelcules to monitor the tryptophan metabolic pathway
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Abstract
Toward improving early detection of melanoma by accurate diagnosis and avoidance of unnecessary surgical excisions of common moles, we are developing noninvasive quantitative spectral fingerprinting of protein expression using Raman spectroscopy within confocally gated volumes of tissue. Our first target is the L-tryptophan catabolism pathway, which is unregulated in the tumor micro-environment and inhibits the immune response that usually is tumor suppressive. The tryptophan pathway is therefore worthy of diagnostic measurement and finding the ratio of L-tryptophan to its metabolites may aid a melanoma diagnosis. We report the intensity of the Raman signal from L-tryptophan and quinolinic acid, which are found during different stages of the tryptophan metabolic pathway.
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Gregory Michel, Alan W. Bigelow, Jamie Harden, James G. Krueger, Daniel S. Gareau, "Raman microbeam spectrometer noninvasively measures biomoelcules to monitor the tryptophan metabolic pathway", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894703 (4 March 2014); doi: 10.1117/12.2040322; https://doi.org/10.1117/12.2040322
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