Raman microbeam spectrometer noninvasively measures biomoelcules to monitor the tryptophan metabolic pathway

Gregory Michel; Alan W. Bigelow; Jamie Harden; James G. Krueger; Daniel S. Gareau

doi:10.1117/12.2040322

4 March 2014 Raman microbeam spectrometer noninvasively measures biomoelcules to monitor the tryptophan metabolic pathway

Gregory Michel; Alan W. Bigelow; Jamie Harden; James G. Krueger; Daniel S. Gareau

Author Affiliations +

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 894703 (2014) https://doi.org/10.1117/12.2040322
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Toward improving early detection of melanoma by accurate diagnosis and avoidance of unnecessary surgical excisions of common moles, we are developing noninvasive quantitative spectral fingerprinting of protein expression using Raman spectroscopy within confocally gated volumes of tissue. Our first target is the L-tryptophan catabolism pathway, which is unregulated in the tumor micro-environment and inhibits the immune response that usually is tumor suppressive. The tryptophan pathway is therefore worthy of diagnostic measurement and finding the ratio of L-tryptophan to its metabolites may aid a melanoma diagnosis. We report the intensity of the Raman signal from L-tryptophan and quinolinic acid, which are found during different stages of the tryptophan metabolic pathway.

Citation Download Citation

Gregory Michel, Gregory Michel, Alan W. Bigelow, Alan W. Bigelow, Jamie Harden, Jamie Harden, James G. Krueger, James G. Krueger, Daniel S. Gareau, Daniel S. Gareau, } "Raman microbeam spectrometer noninvasively measures biomoelcules to monitor the tryptophan metabolic pathway", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894703 (4 March 2014); doi: 10.1117/12.2040322; https://doi.org/10.1117/12.2040322

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years