4 March 2014 Digital holographic microscopy for imaging growth and treatment response in 3D tumor models
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Abstract
While three-dimensional tumor models have emerged as valuable tools in cancer research, the ability to longitudinally visualize the 3D tumor architecture restored by these systems is limited with microscopy techniques that provide only qualitative insight into sample depth, or which require terminal fixation for depth-resolved 3D imaging. Here we report the use of digital holographic microscopy (DHM) as a viable microscopy approach for quantitative, non-destructive longitudinal imaging of in vitro 3D tumor models. Following established methods we prepared 3D cultures of pancreatic cancer cells in overlay geometry on extracellular matrix beds and obtained digital holograms at multiple timepoints throughout the duration of growth. The holograms were digitally processed and the unwrapped phase images were obtained to quantify nodule thickness over time under normal growth, and in cultures subject to chemotherapy treatment. In this manner total nodule volumes are rapidly estimated and demonstrated here to show contrasting time dependent changes during growth and in response to treatment. This work suggests the utility of DHM to quantify changes in 3D structure over time and suggests the further development of this approach for time-lapse monitoring of 3D morphological changes during growth and in response to treatment that would otherwise be impractical to visualize.
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Yuyu Li, Yuyu Li, Ljubica Petrovic, Ljubica Petrovic, Jonathan P. Celli, Jonathan P. Celli, Chandra S. Yelleswarapu, Chandra S. Yelleswarapu, } "Digital holographic microscopy for imaging growth and treatment response in 3D tumor models", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894704 (4 March 2014); doi: 10.1117/12.2040515; https://doi.org/10.1117/12.2040515
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