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4 March 2014 Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy
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Abstract
Regenerative medicine brings a huge application for Mesenchymal stem cells such as Dental Pulp Stem Cells (DPSCs). Confocal Raman microscopy, a non-invasive, label free , real time and high spatial resolution imaging technique is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800-3000 cm-1 region (C-H stretching) and 960 cm-1 peak (phosphate PO4 3-) were collected. In Dental Pulp Stem Cells 21st day differentiated in buffer solution, phosphate peaks ν1 PO4 3- (first vibrational mode) at 960cm-1 and ν2 PO4 3- at 430cm–1 and ν4 PO4 3- at 585cm-1 are obviously present. Confocal Raman microscopy enables the detection of cell differentiation and it can be used to investigate clinical stem cell research.
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H. Salehi, P.-Y. Collart-Dutilleul, C. Gergely, and F. J. G. Cuisinier "Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89470O (4 March 2014); https://doi.org/10.1117/12.2041346
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