4 March 2014 Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy
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Abstract
Regenerative medicine brings a huge application for Mesenchymal stem cells such as Dental Pulp Stem Cells (DPSCs). Confocal Raman microscopy, a non-invasive, label free , real time and high spatial resolution imaging technique is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800-3000 cm-1 region (C-H stretching) and 960 cm-1 peak (phosphate PO4 3-) were collected. In Dental Pulp Stem Cells 21st day differentiated in buffer solution, phosphate peaks ν1 PO4 3- (first vibrational mode) at 960cm-1 and ν2 PO4 3- at 430cm–1 and ν4 PO4 3- at 585cm-1 are obviously present. Confocal Raman microscopy enables the detection of cell differentiation and it can be used to investigate clinical stem cell research.
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H. Salehi, H. Salehi, P.-Y. Collart-Dutilleul, P.-Y. Collart-Dutilleul, C. Gergely, C. Gergely, F. J. G. Cuisinier, F. J. G. Cuisinier, } "Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89470O (4 March 2014); doi: 10.1117/12.2041346; https://doi.org/10.1117/12.2041346
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