Asymmetric-detection time-stretch optical microscopy (ATOM) for high-contrast and high-speed microfluidic cellular imaging

Terence T. W. Wong; Andy K. S. Lau; Matthew Y. H. Tang; Kenneth K. Y. Ho; Kenneth K. Y. Wong; Anderson H. C. Shum; Kevin K. Tsia

doi:10.1117/12.2038952

4 March 2014 Asymmetric-detection time-stretch optical microscopy (ATOM) for high-contrast and high-speed microfluidic cellular imaging

Terence T. W. Wong, Andy K. S. Lau, Matthew Y. H. Tang, Kenneth K. Y. Ho, Kenneth K. Y. Wong, Anderson H. C. Shum, Kevin K. Tsia

Author Affiliations +

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 89471D (2014) https://doi.org/10.1117/12.2038952
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

High-throughput cellular imaging is acclaimed as captivating yet challenging in biomedical diagnostics. We have demonstrated a new imaging modality, asymmetric-detection time-stretch optical microscopy (ATOM), by incorporating a simple detection scheme which is a further advancement in time-stretch microscopy – a viable solution to achieve high-speed and high-throughput cellular imaging. Through the asymmetric-detection scheme in ATOM, the time-stretch image contrast is enhanced through accessing to the phase-gradient information. With the operation in the 1 μm wavelength range, we demonstrate high-resolution and high-contrast cellular imaging in ultrafast microfluidic flow (up to 10 m/s) by ATOM – achieving an imaging throughput equivalent to ~100,000 cells/sec.

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Terence T. W. Wong, Andy K. S. Lau, Matthew Y. H. Tang, Kenneth K. Y. Ho, Kenneth K. Y. Wong, Anderson H. C. Shum, and Kevin K. Tsia "Asymmetric-detection time-stretch optical microscopy (ATOM) for high-contrast and high-speed microfluidic cellular imaging", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471D (4 March 2014); https://doi.org/10.1117/12.2038952

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