4 March 2014 Lens-free digital in-line holographic imaging for wide field-of-view, high-resolution and real-time monitoring of complex microscopic objects
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Abstract
Lens-free inline Holographic Microscopy (LHM) holds great promise for biomedical and industrial applications thanks to its conceptual simplicity. However, the challenge lies in achieving an image quality comparable to conventional microscopes. We demonstrate a high-throughput LHM system that is able to resolve 1.23μm-thin lines on a standard USAF 1951 test target with 1.67μm pixels at the full field-of-view (>29mm2). The system is based on a unique multiwavelength iterative-phase-retrieval method, using customized hardware and real-time post-processing software. We have evaluated our system in experiments ranging from single-cell inspection to in-vitro imaging of stem-cell colonies.
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Richard Stahl, Richard Stahl, Geert Vanmeerbeeck, Geert Vanmeerbeeck, Gauthier Lafruit, Gauthier Lafruit, Roeland Huys, Roeland Huys, Veerle Reumers, Veerle Reumers, Andy Lambrechts, Andy Lambrechts, Chao-Kang Liao, Chao-Kang Liao, Chin-Chun Hsiao, Chin-Chun Hsiao, Masayuki Yashiro, Masayuki Yashiro, Masashi Takemoto, Masashi Takemoto, Tomohisa Nagata, Tomohisa Nagata, Shinichi Gomi, Shinichi Gomi, Kunitada Hatabayashi, Kunitada Hatabayashi, Yasuhiro Oshima, Yasuhiro Oshima, Shigenori Ozaki, Shigenori Ozaki, Naoki Nishishita, Naoki Nishishita, Shin Kawamata, Shin Kawamata, } "Lens-free digital in-line holographic imaging for wide field-of-view, high-resolution and real-time monitoring of complex microscopic objects", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471F (4 March 2014); doi: 10.1117/12.2037619; https://doi.org/10.1117/12.2037619
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