4 March 2014 Video lensfree microscopy of 2D and 3D culture of cells
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Abstract
Innovative imaging methods are continuously developed to investigate the function of biological systems at the microscopic scale. As an alternative to advanced cell microscopy techniques, we are developing lensfree video microscopy that opens new ranges of capabilities, in particular at the mesoscopic level. Lensfree video microscopy allows the observation of a cell culture in an incubator over a very large field of view (24 mm2) for extended periods of time. As a result, a large set of comprehensive data can be gathered with strong statistics, both in space and time. Video lensfree microscopy can capture images of cells cultured in various physical environments. We emphasize on two different case studies: the quantitative analysis of the spontaneous network formation of HUVEC endothelial cells, and by coupling lensfree microscopy with 3D cell culture in the study of epithelial tissue morphogenesis. In summary, we demonstrate that lensfree video microscopy is a powerful tool to conduct cell assays in 2D and 3D culture experiments. The applications are in the realms of fundamental biology, tissue regeneration, drug development and toxicology studies.
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C. P. Allier, C. P. Allier, S. Vinjimore Kesavan, S. Vinjimore Kesavan, J.-G. Coutard, J.-G. Coutard, O. Cioni, O. Cioni, F. Momey, F. Momey, F. Navarro, F. Navarro, M. Menneteau, M. Menneteau, B. Chalmond, B. Chalmond, P. Obeid, P. Obeid, V. Haguet, V. Haguet, B. David-Watine, B. David-Watine, N. Dubrulle, N. Dubrulle, S. Shorte, S. Shorte, B. van der Sanden, B. van der Sanden, C. Di Natale, C. Di Natale, L. Hamard, L. Hamard, D. Wion, D. Wion, M. E. Dolega, M. E. Dolega, N. Picollet-D'hahan, N. Picollet-D'hahan, X. Gidrol, X. Gidrol, J.-M. Dinten, J.-M. Dinten, } "Video lensfree microscopy of 2D and 3D culture of cells", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471H (4 March 2014); doi: 10.1117/12.2038098; https://doi.org/10.1117/12.2038098
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