4 March 2014 Fluorescent cyanine probe for DNA detection and cellular imaging
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In our study, two carbazole-based cyanines, 3,6-bis[2-(1-methylpyridinium)vinyl]-9-methyl carbazole diiodide (A) and 6,6'-bis[2-(1-methylpyridinium)vinyl]-bis(9-methyl-carbazol-3yl)methane diiodide (B) were synthesized and employed as light-up probes for DNA and cell imaging. Both of the cyanine probes possess a symmetric structure and bis-cationic center. The obvious induced circular dichroism signals in circular dichroism spectra reveal that the molecules can specifically interact with DNA. Strong fluorescence enhancement is observed when these two cyanines are bound to DNA. These cyanine probes show high binding affinity to oligonucleotides but different binding preferences to various secondary structures. Confocal microscopy images of fixed cell stained by the probes exhibit strong brightness and high contrast in nucleus with a very low cytoplasmic background.
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Yong-Chao Zheng, Yong-Chao Zheng, Mei-Ling Zheng, Mei-Ling Zheng, Zhen-Sheng Zhao, Zhen-Sheng Zhao, Xuan-Ming Duan, Xuan-Ming Duan, "Fluorescent cyanine probe for DNA detection and cellular imaging", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894722 (4 March 2014); doi: 10.1117/12.2038274; https://doi.org/10.1117/12.2038274

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