New blood markers for staging and prognostics of atherosclerosis

Leticia B. Sicchieri; Mônica Nascimento Silva; Andrea Moreira Monteiro; Antonio Martins Figueiredo Neto; Lilia C. Courrol

doi:10.1117/12.2040240

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4 March 2014 New blood markers for staging and prognostics of atherosclerosis

Leticia B. Sicchieri, Mônica Nascimento Silva, Andrea Moreira Monteiro, Antonio Martins Figueiredo Neto, Lilia C. Courrol

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Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 89472C (2014) https://doi.org/10.1117/12.2040240
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Analysis from the plasmas of rabbits subjected to high-cholesterol diets were performed by fluorescence spectroscopy using three biossensors: Europium-Chlortetracycline (EuCTc), Evans Blue (EB), and Thioflavin T (ThT). For this purpose an animal experimentation was done with New Zealand rabbits divided into two groups: control group of 6 rabbits that received a regular diet for 60 days; and experimental group of 9 rabbits, that were fed with 1% cholesterol for 60 days. The results from spectroscopic analysis have shown that the EuCTc marker emission intensity increases in the presence of plaque formation. The EB emission intensity remained constant for control and experimental groups. The ThT presented an increase in the emission intensity and a modification in the spectra shape with 60 days of diet. The studied biomarkers may not yet be specific in the

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Leticia B. Sicchieri, Mônica Nascimento Silva, Andrea Moreira Monteiro, Antonio Martins Figueiredo Neto, and Lilia C. Courrol "New blood markers for staging and prognostics of atherosclerosis", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89472C (4 March 2014); https://doi.org/10.1117/12.2040240

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