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27 March 2014 Front Matter: Volume 8948
This PDF file contains the front matter associated with SPIE Proceedings Volume 8948, including the Title Page, Copyright Information, Table of Contents, and the Conference Committee listing.

The papers included in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee. Some conference presentations may not be available for publication. The papers published in these proceedings reflect the work and thoughts of the authors and are published herein as submitted. The publisher is not responsible for the validity of the information or for any outcomes resulting from reliance thereon.

Please use the following format to cite material from this book:

Author(s), “Title of Paper,” in Multiphoton Microscopy in the Biomedical Sciences XIV, edited by Ammasi Periasamy, Peter T. C. So, Karsten König, Proceedings of SPIE Vol. 8948 (SPIE, Bellingham, WA, 2014) Article CID Number.

ISSN: 1605-7422

ISBN: 9780819498618

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Conference Committee

Symposium Chairs

  • James G. Fujimoto, Massachusetts Institute of Technology (United States)

  • R. Rox Anderson, Wellman Center for Photomedicine, Massachusetts General Hospital (United States) and Harvard School of Medicine (United States)

Program Track Chairs

  • Ammasi Periasamy, University of Virginia (United States)

  • Daniel L. Farkas, University of Southern California (United States)

Conference Chairs

  • Ammasi Periasamy, University of Virginia (United States)

  • Peter T. C. So, Massachusetts Institute of Technology (United States)

  • Karsten König, Universität des Saarlandes (Germany)

Conference Program Committee

  • Wolfgang Becker, Becker & Hickl GmbH (Germany)

  • Paul J. Campagnola, University of Wisconsin-Madison (United States)

  • Guy C. Cox, The University of Sydney (Australia)

  • Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)

  • Chen-Yuan Dong, National Taiwan University (Taiwan)

  • Kevin W. Eliceiri, University of Wisconsin-Madison (United States)

  • Scott Fraser, California Institute of Technology (United States)

  • Paul M. W. French, Imperial College London (United Kingdom)

  • Hans C. Gerritsen, Utrecht Universiteit (Netherlands)

  • Enrico Gratton, University of California, Irvine (United States)

  • Min Gu, Swinburne University of Technology (Australia)

  • Stefan W. Hell, Max-Planck-Institut für Biophysikalische Chemie (Germany)

  • Satoshi Kawata, Osaka University (Japan)

  • Fu-Jen Kao, National Yang-Ming University (Taiwan)

  • Arnd K. Krueger, Spectra-Physics, a Newport Corporation Brand (United States)

  • Joseph R. Lakowicz, University of Maryland School of Medicine (United States)

  • Steve M. McDonald, Coherent, Inc. (United States)

  • Junle Qu, Shenzhen University (China)

  • Angelika C. Rueck, Universität Ulm (Germany)

  • Steven S. Vogel, National Institutes of Health (United States)

  • Paul W. Wiseman, McGill University (Canada)

  • X. Sunney Xie, Harvard University (United States)

  • Bernhard Zimmermann, Carl Zeiss Jena GmbH (Germany)

  • Warren R. Zipfel, Cornell University (United States)

Session Chairs

  • Keynote Session

  • Ammasi Periasamy, University of Virginia (United States)

  • 1 SHG/THG Microscopy I

    • Francesco Saverio Pavone, European Laboratory for Non-linear Spectroscopy (Italy)

  • 2 SHG/THG Microscopy II

    • Paul J. Campagnola, University of Wisconsin-Madison (United States)

  • 3 Technology Development and Applications I

    • Aisada Uchugonova, Universität des Saarlandes (Germany)

  • 4 Biomedical Applications of Coherent Raman I

    • Annika M. Enejder, Chalmers University of Technology (Sweden)

  • 5 Biomedical Applications of Coherent Raman II

    • Ji-Xin Cheng, Purdue University (United States)

  • 6 Coherent Raman Technical Development I

    • Eric O. Potma, University of California, Irvine (United States)

  • 7 Coherent Raman Technical Development

    • Marcus T. Cicerone, National Institute of Standards and Technology (United States)


    • Peter T. C. So, Massachusetts Institute of Technology (United States)


    • Angelika C. Rueck, Universität Ulm (Germany)

  • 10 Technology Development and Applications II

    • Fu-Jen Kao, National Yang-Ming University (Taiwan)

  • 11 Technology Development and Applications III

    • Karsten König, Universität des Saarlandes (Germany)

  • Poster Session

  • Holly Aaron, University of California, Berkeley (United States)

  • Kevin W. Eliceiri, University of Wisconsin-Madison (United States)

  • Michael Börsch, Friedrich-Schiller-Universität Jena (Germany)

  • Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)

  • Chris Xu, Cornell University (United States)


Multiphoton microscopy has been established as the 3D imaging method of choice for studying biomedical specimens from single cells to whole animals with sub-micron resolution. Two decades have passed since the realization of two-photon laser scanning microscopy. The ever-expanding scope of applications and the continuing instrumental innovations require a forum where new ideas can be exchanged and presented. Our conference at the SPIE Photonics West BiOS 2014 meeting continues to address this need.

This is the 14th year of this conference and we start our conference with three keynote lectures from leaders in the field: Drs. Martin Booth from University of Oxford (United Kingdom), 8948-1: Adaptive optics from microscopy to nanoscopy, Chris Xu, Cornell University (United States), 8948-2: In vivo deep tissue multiphoton imaging, and Karsten König, Saarland University (Germany), 8958-3: Quantitative multiphoton imaging.

For the third year in a row, the conference is extremely pleased to have the JenLab Young Investigator Award, in addition to our regular poster awards. This award is donated by Dr. Karsten König, President and Founder of JenLab GmbH (Germany). The award selection committee includes Drs. Arnd Krueger (NewPort-Spectra Physics), Conor Evans (Harvard University), Paul Campagnola, University of Wisconsin-Madison, and the three conference chairs. The selection process includes the abstract, manuscript and poster presentation. Two finalists are selected for oral presentation after their poster presentation. The two finalists are (1) Dr. Gitanjal Deka, National Yang Ming University (Taiwan), Multiphoton microscopy for skin wound healing study in terms of cellular metabolism and collagen regeneration [8948-71], (2) Ms. Adi Schejter, Nairouz Farah, Shy Shoham, Technion-Israel Institute of Technology (Israel), Two-photon in vivo imaging of retinal microstructures [8948- 75]. Dr. Deka from National Yang Ming University (Taiwan) was selected as the winner of the JenLab Young Investigator Award 2014.

For 14 years in a row, the conference organized poster awards for the students and postdoctoral fellows. The poster award was donated by all the conference sponsors including Becker & Hickl, Boston Electronics, Chroma Technology, Coherent, ISS, Newport-Spectra Physics, Princeton Instruments, Semrock, and Carl Zeiss.

The three poster award winners are

  • 1. Leila B. Mostaco-Guidolin, National Research Council Canada (Canada ), Shedding light into atherosclerosis: a quantitative study of nonlinear optical imaging in tracking plaque development [8948-74]

  • 2. Ming Zhao, College of Optical Sciences, The University of Arizona (United States), Fast multiplexed time-resolved fluorescence microscopy for quantitative time-lapse FRET imaging in cells and deep tissue) [8948-90]

  • 3. Brad Littleton, King’s College London, United Kingdom Hyperspectral imaging via spectral interferometric polarised coherent anti-Stokes Raman scattering) [8948-101]

Some of the most valuable contributions in this volume are articles written by highly experienced practitioners of multi-photon microscopy. They have enumerated the most important considerations in designing multi-photon microscopes and imaging experiments. Further, updates on the state-of-the-art commercial multi-photon microscope systems are presented. This volume also includes articles describing some recent advances in major multi-photon microscope components and applications including laser light sources, ultrafast optics, filters, FRET, FLIM, FCS, Raman, CARS, SRS and CRS microscopy and spectroscopy, single molecule, super-resolution imaging, endoscopy and various scientific and clinical applications.

On a personal note, the Conference Chairs are grateful for the participation of all authors, session chairs and acknowledge the innovation-driven manufacturers (Becker & Hickl GmbH, Boston Electronics, Chroma Technology, Coherent, ISS, JenLab GmbH, Newport-Spectra Physics, Princeton Instruments, Semrock, and Carl Zeiss) for their enthusiastic support in organizing this conference successfully for the last 14 years. We look forward to other exciting conferences in the second decade and welcome your continued participation and support.

Ammasi Periasamy

Peter T. C. So

Karsten König

© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
"Front Matter: Volume 8948", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894801 (27 March 2014);

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