28 February 2014 Quantitative multiphoton imaging
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Abstract
Certified clinical multiphoton tomographs for label-free multidimensional high-resolution in vivo imaging have been introduced to the market several years ago. Novel tomographs include a flexible 360° scan head attached to a mechanooptical arm for autofluorescence and SHG imaging as well as a CARS module. Non-fluorescent lipids and water, mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen can be imaged in vivo with submicron resolution in human skin. Sensitive and rapid detectors allow single photon counting and the construction of 3D maps where the number of detected photons per voxel is depicted. Intratissue concentration profiles from endogenous as well exogenous substances can be generated when the number of detected photons can be correlated with the number of molecules with respect to binding and scattering behavior. Furthermore, the skin ageing index SAAID based on the ratio elastin/collagen as well as the epidermis depth based on the onset of SHG generation can be determined.
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Karsten König, Karsten König, Martin Weinigel, Martin Weinigel, Hans Georg Breunig, Hans Georg Breunig, Aisada Uchugonova, Aisada Uchugonova, } "Quantitative multiphoton imaging", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894804 (28 February 2014); doi: 10.1117/12.2037635; https://doi.org/10.1117/12.2037635
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