28 February 2014 Simultaneous stimulated Raman scattering and higher harmonic generation imaging for liver disease diagnosis without labeling
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Abstract
Nonlinear optical microscopy (e.g., higher harmonic (second-/third- harmonic) generation (HHG), simulated Raman scattering (SRS)) has high diagnostic sensitivity and chemical specificity, making it a promising tool for label-free tissue and cell imaging. In this work, we report a development of a simultaneous SRS and HHG imaging technique for characterization of liver disease in a bile-duct-ligation rat-modal. HHG visualizes collagens formation and reveals the cell morphologic changes associated with liver fibrosis; whereas SRS identifies the distributions of hepatic fat cells formed in steatosis liver tissue. This work shows that the co-registration of SRS and HHG images can be an effective means for label-free diagnosis and characterization of liver steatosis/fibrosis at the cellular and molecular levels.
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Jian Lin, Jian Lin, Zi Wang, Zi Wang, Wei Zheng, Wei Zheng, Zhiwei Huang, Zhiwei Huang, } "Simultaneous stimulated Raman scattering and higher harmonic generation imaging for liver disease diagnosis without labeling", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89480V (28 February 2014); doi: 10.1117/12.2039456; https://doi.org/10.1117/12.2039456
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