28 February 2014 Simultaneous stimulated Raman scattering and higher harmonic generation imaging for liver disease diagnosis without labeling
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Abstract
Nonlinear optical microscopy (e.g., higher harmonic (second-/third- harmonic) generation (HHG), simulated Raman scattering (SRS)) has high diagnostic sensitivity and chemical specificity, making it a promising tool for label-free tissue and cell imaging. In this work, we report a development of a simultaneous SRS and HHG imaging technique for characterization of liver disease in a bile-duct-ligation rat-modal. HHG visualizes collagens formation and reveals the cell morphologic changes associated with liver fibrosis; whereas SRS identifies the distributions of hepatic fat cells formed in steatosis liver tissue. This work shows that the co-registration of SRS and HHG images can be an effective means for label-free diagnosis and characterization of liver steatosis/fibrosis at the cellular and molecular levels.
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Jian Lin, Zi Wang, Wei Zheng, Zhiwei Huang, "Simultaneous stimulated Raman scattering and higher harmonic generation imaging for liver disease diagnosis without labeling", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89480V (28 February 2014); doi: 10.1117/12.2039456; https://doi.org/10.1117/12.2039456
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