28 February 2014 Multimodal microscopy with high resolution spectral focusing CARS
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Abstract
In this work we describe a device that extends capabilities of multiphoton microscopes based on dual wavelength output femtosecond laser sources. CARS with 17cm-1 spectral resolution is experimentally demonstrated. Our approach is based on spectral focusing CARS. For pulse shaping of the pump and Stokes beams we utilize transmission gratings based stretcher. It allows the dispersion of the stretcher to be continuously adjusted in wide range. The best spectral resolution is achieved when the chirp rates in both pump and Stokes beam are matched. The device is automated. Any change in the beam path lengths due to the stretcher adjustment or wavelength tuning is compensated by the delay line. We incorporated into the device a computer controlled beam pointing stabilization system that compensates the beam pointing deviation due to dispersion in the system. High level of automation and computer control makes the operation of the device easy. We present CARS images of several samples that demonstrate high spectral resolution, high contrast and chemical selectivity.
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Tommaso Baldacchini, Tommaso Baldacchini, Ruben Zadoyan, Ruben Zadoyan, } "Multimodal microscopy with high resolution spectral focusing CARS", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894812 (28 February 2014); doi: 10.1117/12.2032729; https://doi.org/10.1117/12.2032729
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