In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy

Nirmal Mazumder; Lu Yun Xiang; Jianjun Qiu; Fu-Jen Kao

doi:10.1117/12.2036651

28 February 2014 In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy

Nirmal Mazumder, Lu Yun Xiang, Jianjun Qiu, Fu-Jen Kao

Author Affiliations +

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 894822 (2014) https://doi.org/10.1117/12.2036651
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We report on measurements and characterization of polarization properties of Second Harmonic (SH) signals using a four-channel photon counting based Stokes polarimeter from type I collagen and starch granules. In this way, the critical polarization parameters including the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP), are extracted from the reconstructed Stokes vector based SH images in a pixel-by-pixel manner. The measurements are further extended to determine the molecular structure and orientation of the samples by varying the polarization states of the incident light and recording the resulting Stokes parameters of the SH signal. The combination of SHG microscopy and Stokes polarimeter hence makes a powerful tool to investigate the structural order of starch granules under water and heating environment.

Citation Download Citation

Nirmal Mazumder, Lu Yun Xiang, Jianjun Qiu, and Fu-Jen Kao "In pixel analysis of molecular structure with Stokes vector resolved second harmonic generation microscopy", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894822 (28 February 2014); https://doi.org/10.1117/12.2036651

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