28 February 2014 Two-photon in vivo imaging of retinal microstructures
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Abstract
Non-invasive fluorescence retinal imaging in small animals is an important requirement in an array of translational vision applications. Two-photon imaging has the potential for long-term investigation of healthy and diseased retinal function and structure in vivo. Here, we demonstrate that two-photon microscopy through a mouse’s pupil can yield high-quality optically sectioned fundus images. By remotely scanning using an electronically tunable lens we acquire highly-resolved 3D fluorescein angiograms. These results provide an important step towards various applications that will benefit from the use of infrared light, including functional imaging of retinal responses to light stimulation.
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Adi Schejter, Nairouz Farah, Shy Shoham, "Two-photon in vivo imaging of retinal microstructures", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894824 (28 February 2014); doi: 10.1117/12.2039375; https://doi.org/10.1117/12.2039375
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