Stepwise multi-photon activation fluorescence reveals a new method of melanoma imaging for dermatologists

Zhenhua Lai; Christine Lian; Jie Ma; Jingyi Yu; Zetong Gu; Milind Rajadhyaksha; Charles A. DiMarzio

doi:10.1117/12.2038312

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28 February 2014 Stepwise multi-photon activation fluorescence reveals a new method of melanoma imaging for dermatologists

Zhenhua Lai, Christine Lian, Jie Ma, Jingyi Yu, Zetong Gu, Milind Rajadhyaksha, Charles A. DiMarzio

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Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 89482D (2014) https://doi.org/10.1117/12.2038312
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Previous research has shown that the stepwise multi-photon activated fluorescence (SMPAF) of melanin, activated by a continuous-wave (CW) mode near infrared (NIR) laser, is a low cost and reliable method of detecting melanin. SMPAF images of melanin in a mouse hair and a formalin fixed mouse melanoma were compared with conventional multiphoton fluorescence microscopy (MPFM) images and confocal reflectance microscopy (CRM) images, all of which were acquired at an excitation wavelength of 920 nm, to further prove the effectiveness of SMPAF in detecting melanin. SMPAF images add specificity for melanin detection to MPFM images and CRM images. Melanin SMPAF can be a promising technology to enable melanoma imaging for dermatologists.

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Zhenhua Lai, Christine Lian, Jie Ma, Jingyi Yu, Zetong Gu, Milind Rajadhyaksha, and Charles A. DiMarzio "Stepwise multi-photon activation fluorescence reveals a new method of melanoma imaging for dermatologists", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482D (28 February 2014); https://doi.org/10.1117/12.2038312

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