28 February 2014 Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin
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Abstract
Multiphoton fluorescence microscopy (MPM) is a method for high resolution, non-invasive investigations of biological tissue. The aim of introducing an annular shaped laser beam is to reduce the ouf-of-focus generated background signal improving imaging of light scattering tissue such as human skin. Simulations show that 50% of the beam radius can be blocked, while preserving the shape of the point spread function. Initial experiments performed on a phantom consisting of fluorescein and fluorescent beads embedded in agar by using a custom built MPM-set up show that by introducing a simple beam blocker to create an annular beam, the background signal is reduced with approximately 5%. Future work will include optimizing the set up, and creating phantoms with more light scattering properties.
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Johan Borglin, Nicholas J. Durr, Stina Guldbrand, Onur Ferhanoglu, Ann-Marie Wennberg, Dag Hanstorp, Adela Ben-Yakar, Marica B. Ericson, "Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482K (28 February 2014); doi: 10.1117/12.2038942; https://doi.org/10.1117/12.2038942
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