28 February 2014 Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin
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Multiphoton fluorescence microscopy (MPM) is a method for high resolution, non-invasive investigations of biological tissue. The aim of introducing an annular shaped laser beam is to reduce the ouf-of-focus generated background signal improving imaging of light scattering tissue such as human skin. Simulations show that 50% of the beam radius can be blocked, while preserving the shape of the point spread function. Initial experiments performed on a phantom consisting of fluorescein and fluorescent beads embedded in agar by using a custom built MPM-set up show that by introducing a simple beam blocker to create an annular beam, the background signal is reduced with approximately 5%. Future work will include optimizing the set up, and creating phantoms with more light scattering properties.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Johan Borglin, Johan Borglin, Nicholas J. Durr, Nicholas J. Durr, Stina Guldbrand, Stina Guldbrand, Onur Ferhanoglu, Onur Ferhanoglu, Ann-Marie Wennberg, Ann-Marie Wennberg, Dag Hanstorp, Dag Hanstorp, Adela Ben-Yakar, Adela Ben-Yakar, Marica B. Ericson, Marica B. Ericson, } "Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482K (28 February 2014); doi: 10.1117/12.2038942; https://doi.org/10.1117/12.2038942

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