In vivo stepwise multi-photon activation fluorescence imaging of melanin in human skin

Zhenhua Lai; Zetong Gu; Saleh Abbas; Jared Lowe; Heidy Sierra; Milind Rajadhyaksha; Charles DiMarzio

doi:10.1117/12.2038310

12 March 2014 In vivo stepwise multi-photon activation fluorescence imaging of melanin in human skin

Zhenhua Lai, Zetong Gu, Saleh Abbas, Jared Lowe, Heidy Sierra, Milind Rajadhyaksha, Charles DiMarzio

Author Affiliations +

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 894919 (2014) https://doi.org/10.1117/12.2038310
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The stepwise multi-photon activated fluorescence (SMPAF) of melanin is a low cost and reliable method of detecting melanin because the activation and excitation can be a continuous-wave (CW) mode near infrared (NIR) laser. Our previous work has demonstrated the melanin SMPAF images in sepia melanin, mouse hair, and mouse skin. In this study, we show the feasibility of using SMPAF to detect melanin in vivo. in vivo melanin SMPAF images of normal skin and benign nevus are demonstrated. SMPAF images add specificity for melanin detection than MPFM images and CRM images. Melanin SMPAF is a promising technology to enable early detection of melanoma for dermatologists.

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Zhenhua Lai, Zhenhua Lai, Zetong Gu, Zetong Gu, Saleh Abbas, Saleh Abbas, Jared Lowe, Jared Lowe, Heidy Sierra, Heidy Sierra, Milind Rajadhyaksha, Milind Rajadhyaksha, Charles DiMarzio, Charles DiMarzio, "In vivo stepwise multi-photon activation fluorescence imaging of melanin in human skin", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 894919 (12 March 2014); doi: 10.1117/12.2038310; https://doi.org/10.1117/12.2038310

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