Two-frequency confocal laser scanning fluorescence microscope for specimen-induced spherical aberration reduction

Yung-Chin Chung; Jheng-Syong Wu; Chien Chou

doi:10.1117/12.2038874

13 March 2014 Two-frequency confocal laser scanning fluorescence microscope for specimen-induced spherical aberration reduction

Yung-Chin Chung, Jheng-Syong Wu, Chien Chou

Author Affiliations +

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 89491M (2014) https://doi.org/10.1117/12.2038874
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

A two-frequency heterodyne confocal laser scanning fluorescence microscope (TF-CLSFM) is setup where a harmonic intensity modulated fluorescence signal is detected coherently. Due to the ability of reduction on refractive index mismatched spherical aberration in TF-CLSFM, it clearly shows that the performance of TF-CLSFM on axial response is better than conventional confocal laser scanning fluorescence microscope (CLSFM) according to the experimental results.

Citation Download Citation

Yung-Chin Chung, Jheng-Syong Wu, and Chien Chou "Two-frequency confocal laser scanning fluorescence microscope for specimen-induced spherical aberration reduction", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89491M (13 March 2014); https://doi.org/10.1117/12.2038874

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