13 March 2014 Two-frequency confocal laser scanning fluorescence microscope for specimen-induced spherical aberration reduction
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Abstract
A two-frequency heterodyne confocal laser scanning fluorescence microscope (TF-CLSFM) is setup where a harmonic intensity modulated fluorescence signal is detected coherently. Due to the ability of reduction on refractive index mismatched spherical aberration in TF-CLSFM, it clearly shows that the performance of TF-CLSFM on axial response is better than conventional confocal laser scanning fluorescence microscope (CLSFM) according to the experimental results.
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Yung-Chin Chung, Jheng-Syong Wu, Chien Chou, "Two-frequency confocal laser scanning fluorescence microscope for specimen-induced spherical aberration reduction", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89491M (13 March 2014); doi: 10.1117/12.2038874; https://doi.org/10.1117/12.2038874
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