12 March 2014 Fast fluorescence holographic microscopy
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Abstract
FINCHSCOPE is a new technology of fluorescence holographic microscopy. It has been successfully applied to recording high-resolution three-dimensional fluorescence images of biological specimens without the need for scanning. In this study, we revealed and analyzed an intrinsic phenomenon, called ghost lens effect, on spatial light modulator which is the core element enabling the incoherent correlation in the FINCHSCOPE. The ghost lens effect can degrade the imaging quality by introducing multiple spherical waves with different focal lengths into the correlation and thus increasing the noise in the recorded holograms.
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Wan Qin, Wan Qin, Xiaoqi Yang, Xiaoqi Yang, Yingying Li, Yingying Li, Xiang Peng, Xiang Peng, Xinghua Qu, Xinghua Qu, Hai Yao, Hai Yao, Bruce Z. Gao, Bruce Z. Gao, } "Fast fluorescence holographic microscopy", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89491W (12 March 2014); doi: 10.1117/12.2041839; https://doi.org/10.1117/12.2041839
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