4 March 2014 Sub-diffraction-limited imaging by photobleaching imprinting microscopy
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Abstract
We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a two-fold improvement over the optical diffraction limit. Unlike other super-resolution imaging techniques, PIM does not require complicated illumination modules or specific fluorescent dyes. PIM is expected to facilitate the conversion of super-resolution imaging into a routine lab tool, making it accessible to a much broader biological research community.
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Liang Gao, Liang Gao, Alejandro Garcia-Uribe, Alejandro Garcia-Uribe, Yan Liu, Yan Liu, Chiye Li, Chiye Li, Lihong Wang, Lihong Wang, } "Sub-diffraction-limited imaging by photobleaching imprinting microscopy", Proc. SPIE 8950, Single Molecule Spectroscopy and Superresolution Imaging VII, 895015 (4 March 2014); doi: 10.1117/12.2038573; https://doi.org/10.1117/12.2038573
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