12 March 2014 Fluorescent imaging over an ultra-large field-of-view of 532 cm2 using a flatbed scanner
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Abstract
We introduce a fluorescent imaging method that is capable of detecting fluorescent micro-particles over an ultra-wide field of view of 19 cm × 28 cm using a modified flatbed scanner. We added a custom-designed absorbing emission filter, a computer controlled two dimensional LED array, and modified the driver of the scanner to maximize the sensitivity, exposure time, and gain for fluorescent detection of micro-objects. This high-throughput fluorescent imaging device used in conjunction with a microfluidic sample holder enables rapid screening of fluorescent micro-objects inside more than 2.2mL of optically dense media (i.e., whole blood) within 5 minutes. The device is sensitive enough to detect fluorescently labeled cells, and generates images that have an effective pixel count of 2.2 Giga-pixels.
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Zoltán S. Göröcs, Yuye Ling, Meng D. Yu, Dimitri Karahalios, Kian Mogharabi, Kenny Lu, Qingshan Wei, Aydogan Ozcan, "Fluorescent imaging over an ultra-large field-of-view of 532 cm2 using a flatbed scanner", Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 89510D (12 March 2014); doi: 10.1117/12.2038602; https://doi.org/10.1117/12.2038602
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