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28 February 2014 Characterization of BxPC3-transplanted mice by hyperspectral autofluorescence imaging and Raman spectroscopy
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Live subcutaneous tumor grown in nude mouse is studied in situ with hyperspectral autofluorescence imaging and Raman spectroscopy. The purpose of the study is to develop methods for characterization of biochemical changing and of histological type of tumor without labeling. The results show that there are site depending variation in the fluorescence and Raman spectra. At the spot in which calcification is in process, Raman spectra showed a strong and specific band at 957 cm-1 due to PO4 species. The autofluosescence image can prove the histological changes based on the NADH and FAD which are major fluorophores in biological tissues. The hyperspectral image is analyzed with principal component analysis and the reconstructed images successfully depicts a different between necrotic and viable part within living subcutaneous tumor.
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Masanori Sawa, Bibin Bintang Andriana, and Hidetoshi Sato "Characterization of BxPC3-transplanted mice by hyperspectral autofluorescence imaging and Raman spectroscopy", Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 89510S (28 February 2014);

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