Method for rapid multidiameter single-fiber reflectance and fluorescence spectroscopy through a fiber bundle

A. Amelink; C. L. Hoy; U. A. Gamm; H. J. C. M. Sterenborg; D. J. Robinson

doi:10.1117/12.2041666

4 March 2014 Method for rapid multidiameter single-fiber reflectance and fluorescence spectroscopy through a fiber bundle

A. Amelink, C. L. Hoy, U. A. Gamm, H. J. C. M. Sterenborg, D. J. Robinson

Author Affiliations +

Proceedings Volume 8952, Biomedical Applications of Light Scattering VIII; 895208 (2014) https://doi.org/10.1117/12.2041666
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We have recently demonstrated a means for quantifying the absorption and scattering properties of biological tissue through multidiameter single-fiber reflectance (MDSFR) spectroscopy. These measurements can be used to correct single-fiber fluorescence (SFF) spectra for the influence of optical properties, enabling quantification of intrinsic fluorescence. In our previous work, we have used a series of pinholes to show that selective illumination and light collection using a coherent fiber bundle can simulate a single solid-core optical fiber with variable diameter for the purposes of MDSFR spectroscopy. Here, we describe the construction and validation of a clinical MDSFR/SFF spectroscopy system that avoids the limitations encountered with pinholes and free-space optics. During one measurement, the new system acquires reflectance spectra at the effective diameters of 200, 600, and 1000 μm, and a fluorescence spectrum at an effective diameter of 1000 μm. From these spectra, we measure the absolute absorption coefficient, μ_a, reduced scattering coefficient, μ’_s, phase function parameter, γ, and intrinsic fluorescence, Qμ^f_a, across the measured spectrum. We validate the system using Intralipid- and polystyrene sphere-based scattering phantoms, with and without the addition of the absorber Evans Blue. Finally, we demonstrate the combined MDSFR/SFF of phantoms with varying concentrations of Intralipid and fluorescein, wherein the scattering properties are measured by MDSFR and used to correct the SFF spectrum for accurate quantification of Qμ^f_a.

Citation Download Citation

A. Amelink, C. L. Hoy, U. A. Gamm, H. J. C. M. Sterenborg, and D. J. Robinson "Method for rapid multidiameter single-fiber reflectance and fluorescence spectroscopy through a fiber bundle", Proc. SPIE 8952, Biomedical Applications of Light Scattering VIII, 895208 (4 March 2014); https://doi.org/10.1117/12.2041666

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KEYWORDS

Tissue optics

Scattering

Optical properties

Luminescence

Reflectivity

Spectroscopy

Fluorescence spectroscopy

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