11 March 2014 Detecting apoptosis in vivo and ex vivo using spectroscopic OCT and dynamic light scattering
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Abstract
We present an in vivo implementation of a multi-parametric technique for detecting apoptosis using optical coherence tomography in a mouse tumor model. Solid tumors were grown from acute myeloid leukemia cells in the hind leg of SCID mice and treated with a single dose of cisplatin and dexamethasone to induce apoptosis. Both spectral features and speckle decorrelation times indicated good consistency between control mice and reasonable agreement with in vitro measurements. The integrated backscatter increased significantly in tumors responding to treatment while the spectral slope and decorrelation time did not show significant changes. This study demonstrates the feasibility of using spectroscopic OCT and dynamic light scattering for treatment monitoring in vivo.
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Golnaz Farhat, Golnaz Farhat, Anoja Giles, Anoja Giles, Adrian Mariampillai, Adrian Mariampillai, Victor X. D. Yang, Victor X. D. Yang, Gregory J. Czarnota, Gregory J. Czarnota, Michael C. Kolios, Michael C. Kolios, } "Detecting apoptosis in vivo and ex vivo using spectroscopic OCT and dynamic light scattering", Proc. SPIE 8952, Biomedical Applications of Light Scattering VIII, 89520K (11 March 2014); doi: 10.1117/12.2040075; https://doi.org/10.1117/12.2040075
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