4 March 2014 Anti-translational research: from the bedside back to the bench for reflectance confocal microscopy
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The reflectance confocal microscope has made translational progress in dermatology. 0.5 micrometer lateral resolution, 0.75mm field-of-view and excellent temporal resolution at ~15 frames/second serve the VivaScope well in the clinic, but it may be overlooked in basic research. This work reviews high spatiotemporal confocal microscopy and presents images acquired of various samples: zebra fish embryo where melanocytes with excellent contrast overly the spinal column, chicken embryo, where myocardium is seen moving at 15 frames/ second, calcium spikes in dendrites (fluorescence mode) just beyond the temporal resolution, and human skin where blood cells race through the artereovenous microvasculature. For an introduction to confocal microscopy, see: http://dangareau.net.s69818.gridserver.com/science/confocal-microscopy
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Daniel Gareau, Daniel Gareau, "Anti-translational research: from the bedside back to the bench for reflectance confocal microscopy", Proc. SPIE 8953, Optical Methods in Developmental Biology II, 89530M (4 March 2014); doi: 10.1117/12.2040504; https://doi.org/10.1117/12.2040504

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