Role of nonspecific binding: a comparison among flow through and flow over assays in nanoporous material

P. Bettotti; N. Kumar; R. Guider; E. Froner; M. Scarpa

doi:10.1117/12.2037700

20 February 2014 Role of nonspecific binding: a comparison among flow through and flow over assays in nanoporous material

P. Bettotti, N. Kumar, R. Guider, E. Froner, M. Scarpa

Author Affiliations +

Proceedings Volume 8954, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI; 89540T (2014) https://doi.org/10.1117/12.2037700
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

In this article we describe the fabrication of free standing n-type porous silicon microcavity (MC) and their properties as liquid sensors. We have optimized the etching recipe to keep both large pore size and high quality factor (Q-factor). Thus the fabricated porous layers have pore size in the range of 40 to 110 nm and are thus compatible with mass transport across the porous layer. We found that MC with a Q-factor of 60 can measure down to 1.1*10^-5 refractive index variations. Furthermore we analyze the role of non specific binding by comparing flow through versus flow over geometries. We compare these two approaches using different techniques and we show that flow over assay systematically overestimates the sensitivity of the device because of an inefficient rinse of the sample. Our work clearly indicates a limit in the reliability of measurements performed in flow over geometry unless specific controls are taken into account.

Citation Download Citation

P. Bettotti, N. Kumar, R. Guider, E. Froner, and M. Scarpa "Role of nonspecific binding: a comparison among flow through and flow over assays in nanoporous material", Proc. SPIE 8954, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XI, 89540T (20 February 2014); https://doi.org/10.1117/12.2037700

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