Bifunctional nanoparticles for surface-enhanced Raman spectroscopy-based leukemia biomarker detection

Dora Mehn; Carlo Morasso; Renzo Vanna; Domitilla Schiumarini; Marzia Bedoni; Fabio Ciceri; Furio Gramatica

doi:10.1117/12.2036174

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3 March 2014 Bifunctional nanoparticles for surface-enhanced Raman spectroscopy-based leukemia biomarker detection

Dora Mehn, Carlo Morasso, Renzo Vanna, Domitilla Schiumarini, Marzia Bedoni, Fabio Ciceri, Furio Gramatica

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Proceedings Volume 8957, Plasmonics in Biology and Medicine XI; 895707 (2014) https://doi.org/10.1117/12.2036174
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The Wilms tumor gene (WT1) is a biomarker overexpressed in more than 90% of acute myeloid leukemia patients. Fast and sensitive detection of the WT1 in blood samples would allow monitoring of the minimal residual disease during clinical remission and would permit early detection of a potential relapse in acute myeloid leukemia. In this work, Surface Enhanced Raman Spectroscopy (SERS) based detection of the WT1 sequence using bifunctional, magnetic core – gold shell nanoparticles is presented. The classical co-precipitation method was applied to generate magnetic nanoparticles which were coated with a gold shell after modification with aminopropyltriethoxy silane and subsequent deposition of gold nanoparticle seeds. Simple hydroquinone based reduction procedure was applied for the shell growing in water based reaction mixture at room temperature. Thiolated ssDNA probes of the WT1 sequence were immobilized as capture oligonucleotides on the gold surface. Malachite green was applied both for testing the amplification performance of the core-shell colloidal SERS substrate and also as label dye of the target DNA sequence. The SERS enhancer efficacy of the core-shell nanomaterial was compared with the efficacy of classical spherical gold particles produced using the conventional citrate reduction method. The core-shell particles were found not only to provide an opportunity for facile separation in a heterogeneous reaction system but also to be superior regarding robustness as SERS enhancers.

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Dora Mehn, Carlo Morasso, Renzo Vanna, Domitilla Schiumarini, Marzia Bedoni, Fabio Ciceri, and Furio Gramatica "Bifunctional nanoparticles for surface-enhanced Raman spectroscopy-based leukemia biomarker detection", Proc. SPIE 8957, Plasmonics in Biology and Medicine XI, 895707 (3 March 2014); https://doi.org/10.1117/12.2036174

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