7 March 2014 Femtosecond optical injection of intact plant cells using a reconfigurable platform
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The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Claire A. Mitchell, Claire A. Mitchell, Stefan Kalies, Stefan Kalies, Tomas Cizmar, Tomas Cizmar, Nicola Bellini, Nicola Bellini, Anisha Kubasik-Thayil, Anisha Kubasik-Thayil, Alexander Heisterkamp, Alexander Heisterkamp, Lesley Torrance, Lesley Torrance, Alison G. Roberts, Alison G. Roberts, Frank J. Gunn-Moore, Frank J. Gunn-Moore, Kishan Dholakia, Kishan Dholakia, "Femtosecond optical injection of intact plant cells using a reconfigurable platform", Proc. SPIE 8972, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV, 89720C (7 March 2014); doi: 10.1117/12.2037784; https://doi.org/10.1117/12.2037784

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